Tuesday, February 5, 2013

EU regulators approve UK's 600 million pound "Green Deal" funding

BRUSSELS (Reuters) - European Union regulators gave approval on Tuesday for Britain's 600 million pounds of public support for the "Green Deal" energy-efficiency scheme.

The European Commission, which acts as regulator for state aid and competition matters in the 27-member European Union said the scheme was a well designed way to achieve a common objective of improving energy efficiency.

"The UK Green Deal allows consumers and businesses to improve the energy efficiency of their buildings without making huge upfront investments," EU Competition Commissioner Joaquin Almunia said in a statement.

Britain's Green Deal permits loans to homeowners to help them pay for efficiency measures such as loft insulation, modern boilers, draught proofing and other materials.

(Rex Merrifield, Brussels newsroom)

Source: http://news.yahoo.com/eu-regulators-approve-uks-600-million-pound-green-135441308.html

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Could Google be working on a unified messaging platform?

Android Central

The screenshot above, while not Android, is extremely interesting regardless because of what it may be showing off. Posted by developer François Beaufort, what's seen here is a Chrome OS desktop with both a new notifications system and more interestingly a new app icon that we've never seen out of Google before. The "rich notifications", which show messages, calls and Google+ activity all in-line and within one pane are displayed in a new Jelly Bean and Google Now design. The new icon, to the right of the Google Drive app in the dock, shows four cascading Google-themed chat boxes.

This screenshot raises a whole lot more questions than it answers, and instantly gets us speculating as to whether or not this new notification system and app are a sign of something bigger than just Chrome OS notifications. Could this finally be a unified messaging service from Google? This could quite easily be just Google+ notifications -- missed hangout calls, Google+ Messenger messages and picture shares -- but we surely hope it's more than that.

Now of course this isn't Android, but it could have a huge impact on Android users if this turns out to be a true unified messaging service from Google. A unified messaging system that brings together Google Voice, Talk, Google+ and others would be a huge step forward for the integration of Google's often-disjointed services.

Source: François Beaufort (Google+); Via: Droid-Life



Source: http://feedproxy.google.com/~r/androidcentral/~3/WOE4OEopgzM/story01.htm

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Monday, February 4, 2013

Not So Obvious Benefits of Buying New ? Rochester Home Builders ...

Prospective home buyers have the choice of two types of houses on the market: resale or new. And we are happy to tell you that area new home builders are seeing a lot of activity at their model homes early this year. A number of builders have reported more than double the interest and activity over recent years which is music to our ears! That?s a great sign for the local economy. Jobs, jobs and more jobs! And that means more money will be spent at area businesses. Yay!

Home buyers planning to buy a brand-new house or condominium often cite energy-efficiency, open layout, a warranty, and being able to select appliances, flooring, paint colors and other design elements as factors driving their choice.

But builders say that buyers can be drawn to a new house for reasons that aren?t so obvious. Below are a few more benefits of a brand-new home that you may not see in the sales brochure.

Building a Community Together


A brand-new community is one of the built-in benefits of many new homes. When families move in to a subdivision at the same time, often lasting bonds of friendship and neighborliness are formed right away. Nobody is the ?new kid on the block,? and many home builders host community block parties in new developments to help owners meet and connect.

Popular amenities like pools, walking trails and courts for tennis and basketball offer additional opportunities for interaction among neighbors of all ages. Often new communities are comprised of home owners in the same stage of life, such as young families or active retirees, so neighbors can get to know each other through carpools, PTA meetings, tennis matches or golf games.

Entertaining

Throwing a party in an older home can be a challenge because smaller, distinct rooms make it difficult to entertain guests in one large space. Builders are responding to today?s home buyer preferences with layouts featuring more open spaces and rooms that flow into each other more easily, like the popular great room. While you are in the kitchen preparing dinner, you can still interact with guests enjoying conversation in the family room without feeling closed off. The feeling of spaciousness in today?s new-home layouts often is enhanced the higher ceilings and additional windows that bringing in more light than you would find in an older home.


A Clean Slate

For some buyers, parking the car in a sparkling-clean garage or being the first to cook a dinner in a brand-new kitchen is part of the appeal of new construction. In addition, you won?t have to spend time stripping dated wallpaper or repainting to suit your own sense of style. You can create your own home d?cor from the get-go!

The advantages of being the first owner of a home extend to the outdoors. Instead of inheriting inconveniently or precariously placed trees, or having to tear up overgrown shrubs, you can design and plant the lawn and garden you want.

Outlets, Outlets Everywhere

Homes built in the 1960?s and earlier were wired much differently than houses today. Builders had no way of anticipating the invention of high-definition televisions, DVRs and computers that we enjoy today ? and the very different electrical requirements they would introduce. New homes can accommodate advanced technologies like structured wiring, security systems and sophisticated lighting plans, and can be tailored to meet the individual home owner?s needs.

Anyone who has ever lived in an older home can also attest to the fact that there are never enough outlets, inside or out! New-home builders plan for the increased number and type of electronics and appliances used by today?s families, so you can safely operate a wine cooler, Christmas lights or your computer.

Article provided by NAHB ? National Association of Home Builders. All member of the RHBA ? the Rochester Home Builders? Association are also members of NAHB.

Source: http://blog.rochesterhomebuilders.com/2013/02/04/not-so-obvious-benefits-of-buying-new/

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Bill Clinton to speak at Ed Koch's funeral in New York City

NEW YORK (AP) ? Ed Koch is being remembered as the quintessential New Yorker ? an admired but tough, colorful former mayor who will be honored at his funeral by former President Bill Clinton.

At the service Monday morning at Manhattan's Temple Emanu-El, mourners will also hear about Koch's other fierce loyalty: Israel. The Israeli consul general is set to speak, along with New York Mayor Michael Bloomberg.

And New York Police Department helicopters are expected to fly over the synagogue in honor of Koch.

Koch was a friend of both Bill and Hillary Rodham Clinton, and was helpful during her successful campaign for the U.S. Senate from New York, according to Koch spokesman George Arzt. Koch also backed Hillary Clinton in her presidential run.

Bill Clinton will serve as a representative for President Barack Obama at the funeral.

Koch died Friday of congestive heart failure at age 88.

Friends from his weekly Greenwich Village luncheon gathering got together on Saturday, two weeks after his last meal with them.

The funeral will be held at one of the nation's most prominent synagogues, a Reform Jewish congregation on Fifth Avenue. Bloomberg is a member, as are comedian Joan Rivers and former New York Gov. Eliot Spitzer.

"I don't want to leave Manhattan, even when I'm gone," he told The Associated Press in 2008 after purchasing a burial plot in Trinity Church Cemetery, at the time the only graveyard in Manhattan that still had space. "This is my home. The thought of having to go to New Jersey was so distressing to me."

Koch led his city for 12 years, with a brash, humor-tinged style that came to personify the New York of the 1980s.

The Democratic mayor is credited with helping save New York from its economic crisis in the 1970s and leading it to financial rebirth. But during his three terms as mayor, he also faced racial tensions and corruption among political allies, as well as the AIDS epidemic, homelessness and urban crime.

In his weekly radio address, Bloomberg called Koch "our most tireless, fearless, and guileless civic crusader."

The mayor said his predecessor's "tough, determined leadership and responsible fiscal stewardship ... helped lift the city out of its darkest days and set it on course for an incredible comeback."

He added, "When someone needed a good kick in the rear, he gave it to them."

Koch lost the Democratic nomination for mayor in 1989 to David Dinkins, who succeeded him.

Koch said he was defeated "because of longevity." In his words, "people get tired of you."

But as the votes were coming in, he said he told himself, "I'm free at last."

Also Monday, U.S. Rep. Carolyn Maloney will announce the renaming of a Manhattan subway station in Koch's honor.

The subway station at East 77th Street and Lexington Avenue will be called "Mayor Ed Koch subway station," according to Maloney.

City officials have introduced legislation to officially rename the station.

Source: http://news.yahoo.com/bill-clinton-speak-ed-kochs-funeral-nyc-202620966.html

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Business Coaching - How Can You Create Win-Win Negotiations ...

Many people think that negotiation is a skill that only sales people need, but the reality is that everyone negotiates every day, both in business and in our daily lives. We negotiate everything from who will walk the dog this evening to what time your boss can expect that critical report to land on his desk.

Some people seem to negotiate with ease, while others feel incredible stress. Here are a few tips to eliminating the stress of negotiations and creating a win-win agreement every time

Negotiate from a position of abundance

Whether you?re negotiating a big deal or just where to go for lunch, always remember that there will be other days and other deals and you will win your fair share. Never go in to a negotiation thinking this is the only deal or the only lunch. In fact, it may help if you consider every negotiation like lunch?there?s always another one tomorrow.

Know what you can live with and what you can?t live without

If you know your bottom line, you know when to walk away from a negotiation and you know what you won?t give up. Knowing this information up front helps you focus and keeps the stress level down.

State clearly what you need or want

Negotiations are much easier if you can be upfront about what you want. Tell the other party what your budget is, or what your constraints are. Let them know what features are important to you. Now you can work together to get as close to what you want as possible.

Ask the other party what they need or want

The ideal win-win negotiation is one where both parties are open and up front about their wants and constraints. Eliminating the gamesmanship that mars so many negotiations eliminates stress and removes the need to ?win.? Oddly enough, when you remove the need for one party to win, it becomes much easier for both parties to win.

Ask yourself what?s fair and then give a little more

Most of us have a bias on our own behalf, so giving up that little extra may actually balance the scale. Even if you give more than you need to, it may pay off over time in repeat business and improved relationships.

Source: http://www.businesscoaching.com/how-can-you-create-win-win-negotiations/

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US military expands its drug war in Latin America

The crew members aboard the USS Underwood could see through their night goggles what was happening on the fleeing go-fast boat: Someone was dumping bales.

When the Navy guided-missile frigate later dropped anchor in Panamanian waters on that sunny August morning, Ensign Clarissa Carpio, a 23-year-old from San Francisco, climbed into the inflatable dinghy with four unarmed sailors and two Coast Guard officers like herself, carrying light submachine guns. It was her first deployment, but Carpio was ready for combat.

Fighting drug traffickers was precisely what she'd trained for.

In the most expensive initiative in Latin America since the Cold War, the U.S. has militarized the battle against the traffickers, spending more than $20 billion in the past decade. U.S. Army troops, Air Force pilots and Navy ships outfitted with Coast Guard counternarcotics teams are routinely deployed to chase, track and capture drug smugglers.

The sophistication and violence of the traffickers is so great that the U.S. military is training not only law enforcement agents in Latin American nations, but their militaries as well, building a network of expensive hardware, radar, airplanes, ships, runways and refueling stations to stem the tide of illegal drugs from South America to the U.S.

According to State Department and Pentagon officials, stopping drug-trafficking organizations has become a matter of national security because they spread corruption, undermine fledgling democracies and can potentially finance terrorists.

U.S. drug czar Gil Kerlikowske, pointing to dramatic declines in violence and cocaine production in Colombia, says the strategy works.

"The results are historic and have tremendous implications, not just for the United States and the Western Hemisphere, but for the world," he said at a conference on drug policy last year.

The Associated Press examined U.S. arms export authorizations, defense contracts, military aid, and exercises in the region, tracking a drug war strategy that began in Colombia, moved to Mexico and is now finding fresh focus in Central America, where brutal cartels mark an enemy motivated not by ideology but by cash.

The U.S. authorized the sale of a record $2.8 billion worth of guns, satellites, radar equipment and tear gas to Western Hemisphere nations in 2011, four times the authorized sales 10 years ago, according to the latest State Department reports.

Over the same decade, defense contracts jumped from $119 million to $629 million, supporting everything from Kevlar helmets for the Mexican army to airport runways in Aruba, according to federal contract data.

Last year $830 million, almost $9 out of every $10 of U.S. law enforcement and military aid spent in the region, went toward countering narcotics, up 30 percent in the past decade.

Many in the military and other law enforcement agencies ? the Drug Enforcement Administration, U.S. Immigration and Customs Enforcement, FBI ? applaud the U.S. strategy, but critics say militarizing the drug war in a region fraught with tender democracies and long-corrupt institutions can stir political instability while barely touching what the U.N. estimates is a $320 billion global illicit drug market.

Congressman Eliot Engel (D-N.Y.), who chaired the U.S. House Subcommittee on the Western Hemisphere for the past four years, says the U.S.-supported crackdown on Mexican cartels only left them "stronger and more violent." He intends to reintroduce a proposal for a Western Hemisphere Drug Policy Commission to evaluate antinarcotics efforts.

"Billions upon billions of U.S. taxpayer dollars have been spent over the years to combat the drug trade in Latin America and the Caribbean," he said. "In spite of our efforts, the positive results are few and far between."

___

At any given moment, 4,000 U.S. troops are deployed in Latin America and as many as four U.S. Navy ships are plying the Caribbean and Pacific coastlines of Central America. U.S. pilots clocked more than 46,400 hours in 2011 flying anti-drug missions, and U.S. agents from at least 10 law enforcement agencies spread across the continent.

The U.S. trains thousands of Latin American troops, and employs its multibillion dollar radar equipment to gather intelligence to intercept traffickers and arrest cartel members.

These work in organized-crime networks that boast an estimated 11,000 flights annually and hundreds of boats and submersibles. They smuggle cocaine from the only place it's produced, South America, to the land where it is most coveted, the United States.

One persistent problem is that in many of the partner nations, police are so institutionally weak or corrupt that governments have turned to their militaries to fight drug traffickers, often with violent results. Militaries are trained for combat, while police are trained to enforce laws.

"It is unfortunate that militaries have to be involved in what are essentially law enforcement engagements," said Frank Mora, the outgoing deputy assistant secretary of defense for Western Hemisphere affairs. But he argues that many governments have little choice.

"We are not going to turn our backs on these governments or these institutions because they've found themselves in such a situation that they have to use their militaries in this way," Mora said.

Mora said the effort is not tantamount to militarizing the war on drugs. He said the Defense Department's role is limited, by law, to monitoring and detection. Law enforcement agents, from the U.S. Coast Guard, Customs and Border Protection or other agencies are in charge of some of the busts, he said.

But the U.S. is deploying its own military. Not only is the Fourth Fleet in the Caribbean, the Pacific and the Atlantic, but the Marines were sent to Guatemala last year and the National Guard is in Honduras.

The Obama Administration sees these deployments as important missions with a worthy payoff. Hundreds of thousands of kilograms (pounds) of cocaine are seized en route to the U.S. every year, and the Defense Department estimates about 850 metric tons of cocaine departed South America last year toward the U.S., down 20 percent in just a year. The most recent U.S. survey found cocaine use fell significantly, from 2.4 million people in 2006 to 1.4 million in 2011.

Aboard the Underwood, the crew of 260 was clear on the mission. The ship's bridge wings bear 16 cocaine "snowflakes" and two marijuana "leaves," awarded to the Underwood by the Coast Guard command to be "proudly displayed" for its successful interdictions.

Standing on the bridge, Carpio's team spotted its first bale of cocaine. And then, after 2 1/2 weeks plying the Caribbean in search of drug traffickers, they spotted another, and then many more.

"In all we found 49 bales," Carpio said in an interview aboard the ship. "It was very impressive to see the bales popping along the water in a row."

Wrapped in black and white tarp, they were so heavy she could barely pull one out of the water. Later, officials said they'd collected $27 million worth of cocaine.

___

The current U.S. strategy began in Colombia in 2000, with an eight-year effort that cost more than $7 billion to stop the flow from the world's top cocaine producer. During Plan Colombia, the national police force, working closely with dozens of DEA agents, successfully locked up top drug traffickers.

But then came "the balloon effect."

As a result of Plan Colombia's pressure, traffickers were forced to find new coca-growing lands in Peru and Bolivia, and trafficking routes shifted as well from Florida to the U.S.-Mexico border.

Thus a $1.6 billion, 4-year Merida Initiative was launched in 2008. Once more, drug kingpins were caught or killed, and as cartels fought to control trafficking routes, increasingly gruesome killings topped 70,000 in six years.

Mexican cartel bosses, feeling the squeeze, turned to Central America as the first stop for South American cocaine, attracted by weaker governments and corrupt authorities.

"Now, all of a sudden, the tide has turned," said Brick Scoggins, who manages the Defense Department's counter-narcotics programs in most of Latin America and the Caribbean. "I'd say northern tier countries of El Salvador, Honduras, Guatemala and Belize have become a key focus area."

The latest iteration is the $165 million Central America Regional Security Initiative, which includes Operation Martillo (Hammer), a year-old U.S.-led mission. The operation has no end date and is focused on the seas off Central America's beach-lined coasts, key shipping routes for 90 percent of the estimated 850 metric tons of cocaine headed to the U.S.

As part of Operation Martillo, 200 U.S. Marines began patrolling Guatemala's western coast in August, their helicopters soaring above villages at night as they headed out to sea to find "narco-submarines" and shiploads of drugs. The troops also brought millions of dollars' worth of computers and intelligence-gathering technology to analyze communications between suspected drug dealers.

Assistant Secretary of State William Brownfield, head of the Bureau of International Narcotics and Law Enforcement Affairs, predicts the balloon effect will play out in Central America before moving to the Caribbean.

The goal, he said, is to make it so hard for traffickers to move drugs to the U.S. that they will eventually opt out of North America, where cocaine use is falling. Traffickers would likely look for easier, more expanding markets, shifting sales to a growing customer base in Europe, Africa and elsewhere in the world.

Brownfield said almost all Peruvian and Bolivian cocaine goes east through Brazil and Argentina and then to Western Europe. Cocaine that reaches North America mostly comes from Colombia, he said, with U.S. figures showing production falling sharply, from 700 metric tons in 2001 to 195 metric tons today ? though estimates vary widely.

When the drug war turns bloody, he said, the strategy is working.

"The bloodshed tends to occur and increase when these trafficking organizations, which are large, powerful, rich, extremely violent and potentially bloody, ... come under some degree of pressure," he said.

Yet the strategy has often backfired when foreign partners proved too inexperienced to fight drug traffickers or so corrupt they switched sides.

In Mexico, for example, the U.S. focused on improving the professionalism of the federal police. But the effort's success was openly questioned after federal police at Mexico City's Benito Juarez International Airport opened fire at each other, killing three.

In August critics were even more concerned when two CIA officers riding in a U.S. Embassy SUV were ambushed by Mexican federal police allegedly working for an organized crime group. The police riddled the armored SUV with 152 bullets, wounding both officers.

The new strategy in Honduras has had its own fits and starts.

Last year, the U.S. Defense Department spent a record $67.4 million on military contracts in Honduras, triple the 2002 defense contracts there well above the $45.6 million spent in neighboring Guatemala in 2012. The U.S. also spent about $2 million training more than 300 Honduran military personnel in 2011, and $89 million in annual spending to maintain Joint Task Force Bravo, a 600-member U.S. unit based at Soto Cano Air Base.

Further, neither the State Department nor the Pentagon could provide details explaining a 2011 $1.3 billion authorization for exports of military electronics to Honduras ? although that would amount to almost half of all U.S. arms exports for the entire Western Hemisphere.

In May, on the other side of the country, Honduran national police rappelled from U.S. helicopters to bust drug traffickers near the remote village of Ahuas, killing four allegedly innocent civilians and scattering locals who were loading some 450 kilograms (close to 1,000 pounds) of cocaine into a boat.

The incident drew international attention and demands for an investigation when the DEA confirmed it had agents aboard the helicopters advising their Honduran counterparts. Villagers spoke of English-speaking commandos kicking in doors and handcuffing locals just after the shooting, searching for drug traffickers.

Six weeks later, townspeople watched in shock as laborers exhumed the first of four muddy graves. At each burial site, workers pulled out the decomposing bodies of two women and two young men, and laid them on tarps.

Forensic scientists conducted their graveside autopsies in the open air, probing for bullet wounds and searching for signs the women had been pregnant, as villagers had claimed.

Government investigators concluded there was no wrongdoing in the raid. In the subsequent months, DEA agents shot and killed suspects they said threatened them in two separate incidents, and the U.S. temporarily suspended the sharing of radar intelligence because the Central American nation's air force shot down two suspected drug planes, a violation of rules of engagement. Support was also withheld for the national police after it was learned that its new director had been tied to death squads.

As the new year begins, Congress is still withholding an estimated $30 million in aid to Honduras, about a third of all the U.S. aid slotted for this year.

But there are no plans to rethink the strategy.

Scoggins, the Defense Department's counter-narcotics manager, said operations in Central America are expected to grow for the next five years.

"It's not for me to say if it's the correct strategy. It's the strategy we are using," said Scoggins. "I don't know what the alternative is."

___

Contributing to this report were Associated Press writers Dario Lopez aboard the USS Underwood in the Caribbean, Garance Burke in San Francisco, Frank Bajak in Lima, Peru, and Alberto Arce in Tegucigalpa, Honduras, along with Romina Ruiz-Goiriena in Guatemala City.

Source: http://news.yahoo.com/us-military-expands-drug-war-latin-america-145202763.html

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Recreating natural complex gene regulation

Recreating natural complex gene regulation [ Back to EurekAlert! ] Public release date: 3-Feb-2013
[ | E-mail | Share Share ]

Contact: Richard Merritt
richard.merritt@duke.edu
919-660-8414
Duke University

DURHAM, N.C. By reproducing in the laboratory the complex interactions that cause human genes to turn on inside cells, Duke University bioengineers have created a system they believe can benefit gene therapy research and the burgeoning field of synthetic biology.

This new approach should help basic scientists as they tease out the effects of "turning on" or "turning off" many different genes, as well as clinicians seeking to develop new gene-based therapies for human disease.

"We know that human genes are not just turned on or off, but can be activated to any level over a wide range. Current engineered systems use one protein to control the levels of gene activation," said Charles Gersbach, assistant professor of biomedical engineering at Duke's Pratt School of Engineering and member of Duke's Institute for Genome Sciences and Policy. "However, we know that natural human genes are regulated by interactions between dozens of proteins that lead to diverse outcomes within a living system.

"In contrast to typical genetics studies that dissect natural gene networks in a top-down fashion, we developed a bottom-up approach, which allows us to artificially simulate these natural complex interactions between many proteins that regulate a single gene," Gersbach said. "Additionally, this approach allowed us to turn on genes inside cells to levels that were not previously possible."

The results of the Duke experiments, which were conducted by Pablo Perez-Pinera, a senior research scientist in Gersbach's laboratory, were published on-line in the journal Nature Methods. The research was supported by the National Institutes of Health, the National Science Foundation, The Hartwell Foundation, and the March of Dimes.

Human cells have about 20,000 genes which produce a multitude of proteins, many of which affect the actions of other genes. Being able to understand these interactions would greatly improve the ability of scientists in all areas of biomedical research. However because of the complexity of this natural system, synthetic biologists create simple gene networks to have precise control over each component. These scientists can use these networks for applications in biosensing, biocomputation, or regenerative medicine, or can use them as models to study the more complex natural systems.

"This new system can be a powerful new approach for probing the fundamental mechanisms of natural gene regulation that are currently poorly understood," Perez-Pinera said. "In this way, we can further the capacity of synthetic biology and biological programming in mammalian systems."

The latest discoveries were made possible by using a new technology for building synthetic proteins known as transcription activator-like effectors (TALEs), which are artificial enzymes that can be engineered to "bind" to almost any gene sequences. Since these TALEs can be easily produced, the researchers were able to make many of them to control specific genes.

"All biological systems depend on gene regulation," Gersbach said. "The challenge facing bioengineering researchers is trying to synthetically recreate processes that occur in nature."

###

Other members of the team were Duke's David Ousterout, Jonathan Brunger, Alicia Farin, Katherine Glass, Farshid Guilak, Gregory Crawford, and Alexander Hartemink.


[ Back to EurekAlert! ] [ | E-mail | Share Share ]

?


AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.


Recreating natural complex gene regulation [ Back to EurekAlert! ] Public release date: 3-Feb-2013
[ | E-mail | Share Share ]

Contact: Richard Merritt
richard.merritt@duke.edu
919-660-8414
Duke University

DURHAM, N.C. By reproducing in the laboratory the complex interactions that cause human genes to turn on inside cells, Duke University bioengineers have created a system they believe can benefit gene therapy research and the burgeoning field of synthetic biology.

This new approach should help basic scientists as they tease out the effects of "turning on" or "turning off" many different genes, as well as clinicians seeking to develop new gene-based therapies for human disease.

"We know that human genes are not just turned on or off, but can be activated to any level over a wide range. Current engineered systems use one protein to control the levels of gene activation," said Charles Gersbach, assistant professor of biomedical engineering at Duke's Pratt School of Engineering and member of Duke's Institute for Genome Sciences and Policy. "However, we know that natural human genes are regulated by interactions between dozens of proteins that lead to diverse outcomes within a living system.

"In contrast to typical genetics studies that dissect natural gene networks in a top-down fashion, we developed a bottom-up approach, which allows us to artificially simulate these natural complex interactions between many proteins that regulate a single gene," Gersbach said. "Additionally, this approach allowed us to turn on genes inside cells to levels that were not previously possible."

The results of the Duke experiments, which were conducted by Pablo Perez-Pinera, a senior research scientist in Gersbach's laboratory, were published on-line in the journal Nature Methods. The research was supported by the National Institutes of Health, the National Science Foundation, The Hartwell Foundation, and the March of Dimes.

Human cells have about 20,000 genes which produce a multitude of proteins, many of which affect the actions of other genes. Being able to understand these interactions would greatly improve the ability of scientists in all areas of biomedical research. However because of the complexity of this natural system, synthetic biologists create simple gene networks to have precise control over each component. These scientists can use these networks for applications in biosensing, biocomputation, or regenerative medicine, or can use them as models to study the more complex natural systems.

"This new system can be a powerful new approach for probing the fundamental mechanisms of natural gene regulation that are currently poorly understood," Perez-Pinera said. "In this way, we can further the capacity of synthetic biology and biological programming in mammalian systems."

The latest discoveries were made possible by using a new technology for building synthetic proteins known as transcription activator-like effectors (TALEs), which are artificial enzymes that can be engineered to "bind" to almost any gene sequences. Since these TALEs can be easily produced, the researchers were able to make many of them to control specific genes.

"All biological systems depend on gene regulation," Gersbach said. "The challenge facing bioengineering researchers is trying to synthetically recreate processes that occur in nature."

###

Other members of the team were Duke's David Ousterout, Jonathan Brunger, Alicia Farin, Katherine Glass, Farshid Guilak, Gregory Crawford, and Alexander Hartemink.


[ Back to EurekAlert! ] [ | E-mail | Share Share ]

?


AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.


Source: http://www.eurekalert.org/pub_releases/2013-02/du-rnc020113.php

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